SPECIFIC ANTIBODIES AGAINST SYNTHETIC PEPTIDES OF SALMONID GTH-I AND GTH-II BETA-SUBUNITS

被引：13

作者：

IKEUCHI, T ^{[1
]}

MOCHIDA, K ^{[1
]}

URA, K ^{[1
]}

ADACHI, S ^{[1
]}

YAMAUCHI, K ^{[1
]}

机构：

[1] HOKKAIDO UNIV,FAC FISHERIES,DEPT BIOL,HAKODATE,HOKKAIDO 041,JAPAN

来源：

ZOOLOGICAL SCIENCE | 1995年 / 12卷 / 03期

关键词：

D O I：

10.2108/zsj.12.317

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Specific polyclonal antibodies against the two salmonid gonadotropins (GTHs) were developed using two synthetic oligopeptides corresponding to GTH I beta-(96-113) and GTH II beta-(107-119), respectively. By Western blot analysis under reducing conditions, anti-GTH I beta-(96-113) detected one specific band corresponding to GTH I beta (17 kDa) and anti-GTH II beta-(107-119) detected one specific band corresponding to GTH II beta (20 kDa). Neither antibody recognized bands under nonreducing conditions. By immunohistochemical analysis, immunoreactive (ir-) I beta-(96-113)and ir-II beta-(107-119)-cells had different regional distributions among the aldehyde fuchsin positive cells and were not stained with an anti-human thyrotropin beta antibody. In salmonid fishes (chum salmon, rainbow trout, brook trout, whitespotted char, dolly varden and huchen), each antibody reacted with distinctly separate hypophyseal cells. However, in nonsalmonid species, and even in the salmoniform fish, the ayu (Plecoglossus altivelis), no pituitary cell was stained with these antibodies. These data indicate that these antibodies are useful tools for studying the change and the function of GTHs in salmonid fishes.

引用

页码：317 / 323

页数：7

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