A SUBFAMILY 2 HOMO-DIMERIC GLUTATHIONE-S-TRANSFERASE MYRS-MYRS OF CLASS-THETA IN MOUSE-LIVER CYTOSOL

被引：11

作者：

HIRATSUKA, A ^{[1
]}

KANAZAWA, M ^{[1
]}

NISHIYAMA, T ^{[1
]}

OKUDA, H ^{[1
]}

OGURA, K ^{[1
]}

WATABE, T ^{[1
]}

机构：

[1] TOKYO UNIV PHARM & LIFE SCI,SCH PHARM,DEPT DRUG METAB & MOLEC TOXICOL,HACHIOJI,TOKYO 19203,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 212卷 / 03期

关键词：

D O I：

10.1006/bbrc.1995.2032

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A homo-dimeric subfamily 2 glutathione (GSH) S-transferase (GST) mYrs-mYrs of the class theta was isolated from mouse liver cytosol and purified to homogeneity. The first 28 N-terminal amino acid sequence of the GST was completely identical to that of rat subfamily 2 GST Yrs-Yrs of the class theta. GST mYrs-mYrs cross-reacted with anti-rat GST Yrs-anti-sera but not with anti-sera raised against rat GSTs Ya-Ya (alpha), Yb1-Yb1 (mu), and Yp-Yp (pi) and represented more than 95% of the mouse liver cytosolic GST activity to scavenge the reactive sulfate ester 5-sulfoxymethylchrysene of the potent carcinogen 5-hydroxymethylchrysene. The mouse class theta GST had little activity toward 1-chloro-2,4-dinitrobenzene and was unretainable on GSH and an S-hexyl-GSH affinity columns. GST mYrs-mYrs had a much higher GSH peroxidase activity toward fatty acid hydroperoxides than did the other classes of mouse GSTs. (C) 1995 Academic Press, Inc.

引用

页码：743 / 750

页数：8

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