CHARACTERIZATION OF THE SPOIVB AND RECN LOCI OF BACILLUS-SUBTILIS

被引:29
作者
VANHOY, BE [1 ]
HOCH, JA [1 ]
机构
[1] SCRIPPS RES INST, DEPT MOLEC & EXPTL MED,DIV CELLULAR BIOL, LA JOLLA, CA 92037 USA
关键词
D O I
10.1128/jb.172.3.1306-1311.1990
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Two independent genes, recN and spoIVB, along with their respective promoter and termination regions, were discovered and sequenced in the 3.4-kilobase region between the ahrC and spoOA genes at map position 216 in the Bacillus subtilis chromosome map. The gene encoding a 576-amino-acid protein, which maintains a high homology with Escherichia coli recN gene product, was adjacent to ahrC. The sequence revealed a 64,472-dalton polypeptide which contained a conserved ATP-binding site and possible lexA-type regulatory binding sequences in its promoter region. A second open reading frame identified as the spoIVB gene was directly downstream of recN. It consisted of 1,275 nucleotides which coded for a 425-amino-acid polypeptide with a molecular weight of 45.976. Phenotypic, genetic, and transcriptional analyses confirmed that this gene was spoIVB. Although no chloroform-resistant spores were produced by spoIVB-inactivated strains, under microscopic examination, phase-gray forespores were visible. The spoIVB165 mutation was localized to a 200-base-pair region in the amino-terminal portion of the polypeptide. spoIVB was not transcribed until hour 2 of sporulation in wild-type B. subtilis cells, as determined by β-galactosidase activity assays from lacZ transcriptional fusion constructions. We found no amino acid sequence homology between the spoIVB gene product and other known bacterial proteins.
引用
收藏
页码:1306 / 1311
页数:6
相关论文
共 26 条
[1]   REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS [J].
ANAGNOSTOPOULOS, C ;
SPIZIZEN, J .
JOURNAL OF BACTERIOLOGY, 1961, 81 (05) :741-&
[2]  
BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513
[3]   SUPERCOIL SEQUENCING - A FAST AND SIMPLE METHOD FOR SEQUENCING PLASMID DNA [J].
CHEN, EY ;
SEEBURG, PH .
DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1985, 4 (02) :165-170
[4]   SPORULATION IN BACILLUS-SUBTILIS - GENETIC ANALYSIS OF OLIGOSPOROGENOUS MUTANTS [J].
COOTE, JG .
JOURNAL OF GENERAL MICROBIOLOGY, 1972, 71 (JUN) :17-&
[5]  
COOTE JG, 1972, J GEN MICROBIOL, V71, P1
[6]   TRANSCRIPTION OF BACILLUS-SUBTILIS SUBTILISIN AND EXPRESSION OF SUBTILISIN IN SPORULATION MUTANTS [J].
FERRARI, E ;
HENNER, DJ ;
PEREGO, M ;
HOCH, JA .
JOURNAL OF BACTERIOLOGY, 1988, 170 (01) :289-295
[7]   CHARACTERIZATION OF THE SPO0A LOCUS AND ITS DEDUCED PRODUCT [J].
FERRARI, FA ;
TRACH, K ;
LECOQ, D ;
SPENCE, J ;
FERRARI, E ;
HOCH, JA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (09) :2647-2651
[8]   CLUSTAL - A PACKAGE FOR PERFORMING MULTIPLE SEQUENCE ALIGNMENT ON A MICROCOMPUTER [J].
HIGGINS, DG ;
SHARP, PM .
GENE, 1988, 73 (01) :237-244
[9]   SELECTION OF CELLS TRANSFORMED TO PROTOTROPHY FOR SPORULATION MARKERS [J].
HOCH, JA .
JOURNAL OF BACTERIOLOGY, 1971, 105 (03) :1200-&
[10]   GENETICS OF BACTERIAL SPORULATION [J].
HOCH, JA .
ADVANCES IN GENETICS, 1976, 18 :69-98