DYE-LABELED SUBSTRATES FOR THE ASSAY AND DETECTION OF CHITINASE AND LYSOZYME ACTIVITY

被引：241

作者：

WIRTH, SJ ^{[1
]}

WOLF, GA ^{[1
]}

机构：

[1] UNIV GOTTINGEN, INST PFLANZENPATHOL & PFLANZENSCHUTZ, GRISEBACHSTR 6, W-3400 GOTTINGEN, GERMANY

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1990年 / 12卷 / 3-4期

关键词：

CHITINASE ASSAY; CHITIN DERIVATIVE; PLATE-CLEARING ASSAY; REMAZOL BRILLIANT VIOLET-5R;

D O I：

10.1016/0167-7012(90)90031-Z

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A carboxymethyl-substituted soluble chitin and a reprecipitated colloidal chitin derivative were covalently linked with Remazol Brilliant Violet 5R. Both conjugates are new dye-labelled substrates suitable for the assay and detection of chitinase and lysozyme activity. A reliable colorimetric microassay adapted to microtitre plates was established, based on the precipitability of carboxymethyl-chitin-Remazol Brilliant Violet 5R from buffered solutions with hydrochloric acid. Further more, sensitive plate-clearing assays were developed for the isolation and detection of chitinolytic microbial populations, and for the screening of pure cultures.

引用

页码：197 / 205

页数：9

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