ISOLATION AND PROPERTIES OF THE MEMBRANE-INTEGRATED PART OF THE ATP-SYNTHASE FROM CHLOROPLASTS, CF0

被引：9

作者：

GROTJOHANN, I

GRABER, P

机构：

[1] UNIV STUTTGART,INST BIOL,PFAFFENWALDRING 57,W-7000 STUTTGART 80,GERMANY

[2] TECH UNIV BERLIN,MAX VOLMER INST,W-1000 BERLIN 12,GERMANY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1017卷 / 02期

关键词：

ATP-synthase; ATPase; Chloroplast; Ion translocation; Proton-translocating ATPase; Reconstitution;

D O I：

10.1016/0005-2728(90)90149-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ATP-synthase from chloroplasts, CF0F1, was isolated and purified and finally reconstituted into liposomes. The CF1 part was solubilized from the CF0F1 proteoliposomes by treatment with NaBr. The resulting CF0 protecoliposomes showed a proton conductivity of 16H+/CF0 per s driven by a K+/valinomycin diffusion potential. CF0 can be removed from the liposomes by treatment with octyl glucoside. SDS-gel electrophoresis shows that CF0 contains four different subunits, I, II, III, IV. © 1990.

引用

页码：177 / 180

页数：4

共 16 条

[1]

BOEKEMA EJ, 1988, Z NATURFORSCH C, V43, P219

[2] A 6TH SUBUNIT OF ATP SYNTHASE, AN F0 COMPONENT, IS ENCODED IN THE PEA CHLOROPLAST GENOME [J].