CATALYTIC EFFECTS OF PEROXIDASE-LIKE METALLOPORPHYRINS ON THE FLUORESCENCE REACTION OF HOMOVANILLIC-ACID WITH HYDROGEN-PEROXIDE

The catalytic effects of peroxidase-like metalloporphyrins (Me-P) on the fluorescence reaction of homovanillic acid with hydrogen peroxide have been studied. These metalloporphyrins are the complexes of Mn with tetrakis(carboxyphenyl)porphyrin (TPPC) and trikis(sulfophenyl)porphyrin(TPPS3), Fe, Co, Ni, Cu, Zn, Ag and Sn with tetrakis(sulfophenyl)porphyrin(TPPS4), and Rh, Pt and Pd with tetrakis(N-methylpyridiniumyl)porphyrin-(TMPyP) and hemin. The complexes of Mn, Fe, Co, Rh and Pt with porphyrins catalyzed the formation of the fluorescence product, while the complexes of Ni, Cu, Zn, Ag, Sn and Pd did not. Traces of hydrogen peroxide and glucose can be determined using the metalloporphyrins. The characteristics of peroxidase-like metalloporphyrins have been compared with those of horseradish peroxidase (HRP).