INSITU HYBRIDIZATION BANDING OF HUMAN-CHROMOSOMES WITH ALU-PCR PRODUCTS - A SIMULTANEOUS KARYOTYPE FOR GENE-MAPPING STUDIES

被引：128

作者：

BALDINI, A ^{[1
]}

WARD, DC ^{[1
]}

机构：

[1] INST MOLEC GENET,CNR,PORTO CONTE RES & TRAINING LABS,SASSATI,ITALY

来源：

GENOMICS | 1991年 / 9卷 / 04期

关键词：

D O I：

10.1016/0888-7543(91)90374-N

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Polymerase chain reaction products generated from a single Alu primer and human genomic DNA produce a distinct and highly reproducible R-banding pattern when hybridized to metaphase chromosome spreads. Individual chromosomes can be readily identified and karyotyped. Compared to conventional fluorescence banding on heat-denatured chromosomes, the in situ hybridization banding (ISHB) shows high contrast and definition. We demonstrate that this banding method can be employed effectively in double-labeling experiments for the rapid and simultaneous assignment of probes to specific chromosomal bands. Since virtually any fluorochrome can be used to delineate chromosomal bands, ISHB should provide added flexibility for multicolor mapping strategies. © 1991.

引用

页码：770 / 774

页数：5

共 25 条

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