HUMAN-IMMUNODEFICIENCY-VIRUS - GACPAT AND GACELISA AS DIAGNOSTIC-TESTS FOR ANTIBODIES IN URINE

被引:15
作者
STERNE, JAC
TURNER, AC
CONNELL, JA
PARRY, JV
FINE, PEM
PONNIGHAUS, JM
NYASULU, S
MKANDWIRE, PK
机构
[1] CENT PUBL HLTH LAB,PUBL HLTH LAB SERV,VIRUS REF LAB,LONDON NW9 5HT,ENGLAND
[2] LEPRA EVALUAT PROJECT,CHILUMBA,MALAWI
关键词
D O I
10.1016/0035-9203(93)90480-E
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
Testing for antibodies to human immunodeficiency virus (HIV) in urine may be of use in epidemiological studies. We collected 336 paired urine and serum samples from subjects in Karonga District, northern Malawi: 86 (25.6%) of the serum samples were HIV positive. Serum results were compared with those from immunoglobulin (Ig) G antibody-capture particle adherence tests (GACPAT) and IgG antibody-capture enzyme-linked immunosorbent assay (GACELISA) on the corresponding urine samples performed independently in 2 laboratories. The minimum observed relative sensitivity and specificity Of GACPAT were 96.5% and 98.8% respectively; the specificity could be raised by using a protocol involving re-testing of reactive samples to determine end-point titre. For GACELISA, the observed relative sensitivity and specificity were 98-8% and 99.2% respectively. Such assays may be useful either as a primary screen in populations where urine samples are considerably easier to obtain than serum samples, or as an alternative test for individuals unwilling to provide a serum sample.
引用
收藏
页码:181 / 183
页数:3
相关论文
共 7 条
  • [1] BARBARA JAJ, 1989, MED LAB SCI, V46, P135
  • [2] ACCURATE ASSAYS FOR ANTI-HIV IN URINE - PRELIMINARY-REPORT
    CONNELL, JA
    PARRY, JV
    MORTIMER, PP
    DUNCAN, RJS
    MCLEAN, KA
    JOHNSON, AM
    HAMBLING, MH
    BARBARA, J
    FARRINGTON, CP
    [J]. LANCET, 1990, 335 (8702) : 1366 - 1369
  • [3] LEPROSY IN MALAWI .2. BACKGROUND, DESIGN AND PROSPECTS OF THE KARONGA PREVENTION TRIAL, A LEPROSY VACCINE TRIAL IN NORTHERN MALAWI
    FINE, PEM
    PONNIGHAUS, JM
    [J]. TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1988, 82 (06) : 810 - 817
  • [4] MORTIMER PP, 1991, REV MED VIROL, V1, P73
  • [5] AN IMMUNOGLOBULIN-G ANTIBODY CAPTURE PARTICLE-ADHERENCE TEST (GACPAT) FOR ANTIBODY TO HIV-1 AND HTLV-I THAT ALLOWS ECONOMICAL LARGE-SCALE SCREENING
    PARRY, JV
    MORTIMER, PP
    [J]. AIDS, 1989, 3 (03) : 173 - 176
  • [6] PONNIGHAUS JM, 1991, INT J LEPROSY, V59, P221
  • [7] SHAW MA, 1991, LEPROSY REV, V62, P87