EFFECT OF INCREASED PPGPP CONCENTRATION ON DNA-REPLICATION OF DIFFERENT REPLICONS IN ESCHERICHIA-COLI

被引：24

作者：

HERMAN, A ^{[1
]}

WEGRZYN, G ^{[1
]}

机构：

[1] UNIV GDANSK,DEPT MOLEC BIOL,MOLEC GENET LAB,PL-80822 GDANSK,POLAND

来源：

JOURNAL OF BASIC MICROBIOLOGY | 1995年 / 35卷 / 01期

关键词：

D O I：

10.1002/jobm.3620350110

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The plasmids harbouring the relA gene under an inducible promoter allowed us to increase the guanosine 5'-diphosphate-3'-diphosphate (ppGpp) concentration in Escherichia coli cells without any starvation and thus, to directly investigate the effect of ppGpp on DNA replication. We studied all types of replicons which were investigated previously in amino acid-starved bacteria and found that ColE1, oriC, lambda plasmid and pSC101 but not RK2 replicons are sensitive to high ppGpp level. To our knowledge, this paper presents the first direct evidence that replication of most, but not all, replicons is dependent on ppGpp concentration and thus, is under stringent control.

引用

页码：33 / 39

页数：7

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