CONTINUOUS PRODUCTION OF AVIAN SARCOMA VIRUS B77 BY RAT TUMOUR CELLS IN TISSUE CULTURE

被引：29

作者：

SIMKOVIC, D

POPOVIC, M

SVEC, J

GROFOVA, M

VALENTOVA, N

机构：

[1] London, N W. 7, Burtonhole Lane, Mill Hill

来源：

INTERNATIONAL JOURNAL OF CANCER | 1969年 / 4卷 / 01期

关键词：

D O I：

10.1002/ijc.2910040111

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The rat tumour RBI, previously induced by injection of fowl sarcoma B77 tissue into newborn rats, was adapted to long‐term growth in tissue culture. Two cell lines, 16B177 and 17B177, were derived from the tumour. Both cell lines were capable of continuous production and release of infectious B77 virus over a period of more than two years' in vitro cultivation. The virus present in cell‐free tissue culture media induced characteristic sarcomas upon inoculation into young chicks. Preliminary chromosomal analysis of the uncloned population of 17BI77 cells confirmed their rat origin and revealed that the hypotriploid stem‐line predominates, being represented by 59 chromosomes. Copyright © 1969 Wiley‐Liss, Inc., A Wiley Company

引用

页码：80 / +

页数：1

共 15 条

[1]

ALTANER C, 1966, J NATL CANCER I, V37, P745

[2] PLAQUE FORMATION AND ISOLATION OF PURE LINES WITH POLIOMYELITIS VIRUSES [J].