STUDIES ON AUXIN PROTECTORS .13. MASKING OF PEROXIDASE-CATALYZED OXIDATION OF IAA IN VIGNA

被引：24

作者：

STONIER, T ^{[1
]}

STASINOS, S ^{[1
]}

MURTHYREDDY, KBS ^{[1
]}

机构：

[1] APS COLL, BANGALORE 560019, INDIA

来源：

PHYTOCHEMISTRY | 1979年 / 18卷 / 01期

关键词：

auxin protector: IAA-oxidase; cowpea; Leguminosae; peroxidase; phenylpropanoids; Vigna sinensis;

D O I：

10.1016/S0031-9422(00)90908-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Partially purified enzyme preparations of extracts of Vigna seedlings exhibited guaiacol-oxidase activity but not IAA-oxidase activity. However, by ageing the enzyme preparations, or by treating them with H2O2, it was possible to unmask IAA-oxidase activity. Gel filtration of Vigna extracts on Sepharose yielded separate peaks for IAA-oxidase, guaiacol-oxidase and auxin protectors. The appearance of a separate IAA-oxidase peak reflected the overlap of peroxidase and protector; the apparent difference in the migration rate of IAA-oxidase and guaiacol-oxidase activity proved to be an artifact. The data imply that previous reports of differences between peroxidase and IAA oxidase need to be reinvestigated to rule out the possible effect of contamination by endogenous, high MW auxin protectors. A rapid method for removing most of the auxin protectors and thereby unmasking IAA-oxidase activity is described. © 1979.

引用

页码：25 / 28

页数：4

共 28 条

[1]

BASTIN M, 1964, B SOC R SCI LIEGE, V11, P678

[2]

ENDO T, 1968, PLANT CELL PHYSIOL, V9, P333

[3] FLAVOPROTEIN AND PEROXIDASE AS COMPONENTS OF THE INDOLEACETIC ACID OXIDASE SYSTEM OF PEAS [J].