K-CHANNEL BLOCKING-DRUGS INDUCE HISTAMINE-RELEASE AND CA-45 UPTAKE IN ISOLATED MAST-CELLS

被引:16
作者
ELENO, N
BOTANA, L
ESPINOSA, J
机构
[1] UNIV SANTIAGO DE COMPOSTELA,DEPT PHARMACOL,SANTIAGO,SPAIN
[2] UNIV SANTIAGO DE COMPOSTELA,DEPT PHYSIOL,SANTIAGO,SPAIN
来源
INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY | 1990年 / 92卷 / 02期
关键词
D O I
10.1159/000235208
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Histamine secretion and 45Ca uptake processes were studied in mast cells treated with four K+ channel blocking drugs in physiological saline and in media containing different ionic concentrations. Quinine, 4-aminopyridine and sparteine were effective as histamine-releasing agents when mast cells were incubated in physiologic saline solution. The dose-response profile obtained was in the range of 0.1-0.5 mM for quinine, 1-10 for 4-aminopyridine and 0.5-5 raM for sparteine and did not show significant differences between purified and unpurified mast cells. By contrast, tetraethylammonium (1-100 mM) did not induce histamine release. The presence of high K+ or Rb+concentrations in the medium (Tris-K+ or Tris-Rb+, both at 150 m M) displaced the profile obtained to the right in cells stimulated with 4-aminopyridine or sparteine, but abolished histamine release induced by quinine. Additionally, all three K+ channel blockers increased 45Ca uptake in mast cells. The exact mechanism of the action of K+ channel blockers on mast cells is unknown. However, the fact that the drugs used were effective as histamine-releasing and 45Ca uptake promoters suggests both that mast cells might be endowed with a K+ channel activity and that the blockade of this should open certain calcium channels, leading to elevated intracellular Ca2+ levels which in turn activate mast cell secretion. © 1990 S. Karger AG, Basel.
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页码:162 / 167
页数:6
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