A SINGLE HUMAN MONOCLONAL-ANTIBODY THAT CONFERS TOTAL PROTECTION FROM TETANUS

被引：16

作者：

ARUNACHALAM, B ^{[1
]}

GHOSH, S ^{[1
]}

TALWAR, GP ^{[1
]}

RAGHUPATHY, R ^{[1
]}

机构：

[1] NATL INST IMMUNOL,NEW DELHI 110067,INDIA

来源：

HYBRIDOMA | 1992年 / 11卷 / 02期

关键词：

D O I：

10.1089/hyb.1992.11.165

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Protective human monoclonal antibodies (HuMAbs) are superior to hyperimmune sera and murine monoclonal antibodies as far as human immunotherapy is concerned. In this report, we describe the successful generation of triomas secreting HuMAbs to tetanus toxin (tt). LymphobLastoid cell lines secreting anti-tt antibodies were stabilized by back-fusion with a mouse x human heterohybrid myeloma partner, SBC-H20. One of the antibodies so produced, confers total protection of mice from tetanus, unlike a few recent reports where only partial protection (delay in the onset of tetanus) was achieved with single HuMAbs. Experiments to localize the neutralizing epitope(s) of the toxin using the protective monoclonal antibodies revealed that the antibody recognizes a conformational determinant that is destroyed on SDS-treatment. Preliminary studies show that Fab preparations of the protective antibody are capable of neutralizing tetanus toxin, suggesting that it might be possible to clone and express the Fab in a stable vector for Large scale production.

引用

页码：165 / 179

页数：15

共 60 条

[1] THE TETANUS TOXIN LIGHT CHAIN INHIBITS EXOCYTOSIS [J].