IMMUNOHISTOCHEMICAL STUDY TO DEMONSTRATE SARCOCYSTIS-NEURONA IN EQUINE PROTOZOAL MYELOENCEPHALITIS

被引：48

作者：

HAMIR, AN

MOSER, G

GALLIGAN, DT

DAVIS, SW

GRANSTROM, DE

DUBEY, JP

机构：

[1] University of Pennsylvania School of Veterinary Medicine, New Bolton Center, Kennett Square, PA 19348

[2] Zoonotic Diseases Laboratory, Livestock and Poultry Sciences Institute, ARS, USDA, Beltsville

[3] Department of Veterinary Science, University of Kentucky, Lexington

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 1993年 / 5卷 / 03期

关键词：

D O I：

10.1177/104063879300500320

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A 5-year (1985-1989) retrospective immunohistochemical study was conducted using an avidin-biotin complex (ABC) immunoperoxidase method to demonstrate Sarcocystis neurona in histologically suspect cases of equine protozoal myeloencephalitis (EPM). Primary antibodies against S. neurona and S cruzi were utilized for the ABC technique. The findings were compared with those from cases in which the organisms were detected by examination of hematoxylin and eosin (HE)-stained neuronal sections. HE-stained sections detected the presence of the organisms in 20% of the suspect cases; whereas the ABC technique confirmed the presence of S. neurona in 51% and 67% of the cases by S. neurona and S. cruzi antibodies, respectively. A review of clinical case histories showed that 21/47 (45%) of the EPM horses with parasites in the tissue sections had prior treatment with antiprotozoal drugs and/or steroids. Using the test results of S. neurona and S. cruzi as a standard reference, HE test sensitivity based on examination of up to 30 neuronal sections per case was only 25%, and test specificity was 91%.

引用

页码：418 / 422

页数：5

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