POLYMERASE CHAIN REACTION-MEDIATED CLONING AND IN-VITRO TRANSLATION OF THE GENES-CODING FOR THE STRUCTURAL PROTEINS OF HOG-CHOLERA VIRUS

被引：14

作者：

MUYLDERMANS, G ^{[1
]}

SANGABRIEL, MC ^{[1
]}

CAIJ, A ^{[1
]}

DESMET, A ^{[1
]}

HAMERS, R ^{[1
]}

机构：

[1] NATL INST VET RES, BRUSSELS, BELGIUM

来源：

ARCHIVES OF VIROLOGY | 1993年 / 132卷 / 3-4期

关键词：

D O I：

10.1007/BF01309551

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

After amplification by PCR, the 5' region of the genome of hog cholera virus (HCV) strain Alfort 187 was cloned and sequenced. The nucleotide and deduced amino acid sequences were compared with the ones of other pestiviruses. By in vitro translation experiments we were able to demonstrate the protease activity of the p 20 protein of HCV.

引用

页码：429 / 435

页数：7

共 21 条

[1] PESTIVIRUS BOVINE VIRAL DIARRHEA VIRUS POLYPEPTIDES - IDENTIFICATION OF NEW PRECURSOR PROTEINS AND ALTERNATIVE CLEAVAGE PATHWAYS
AKKINA, RK
[J]. VIRUS RESEARCH, 1991, 19 (01) : 67 - 82
[2] HIGH TITER HOG-CHOLERA VIRUS PRODUCTION ON CYTODEX-3 MICROCARRIER CULTURES
CAIJ, A
DESMET, A
DUBOIS, N
KOENEN, F
[J]. ARCHIVES OF VIROLOGY, 1989, 105 (1-2) : 113 - 118
[3] MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF THE PESTIVIRUS BOVINE VIRAL DIARRHEA VIRUS
COLLETT, MS
LARSON, R
GOLD, C
STRICK, D
ANDERSON, DK
PURCHIO, AF
[J]. VIROLOGY, 1988, 165 (01) : 191 - 199
[4] PROTEINS ENCODED BY BOVINE VIRAL DIARRHEA VIRUS - THE GENOMIC ORGANIZATION OF A PESTIVIRUS
COLLETT, MS
LARSON, R
BELZER, SK
RETZEL, E
[J]. VIROLOGY, 1988, 165 (01) : 200 - 208
[5] COLLETT MS, 1991, ARCH VIROL, P19
[6] Darbyshire J. H., 1960, Veterinary Record, V72, P331
[7] MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF A PESTIVIRUS GENOME, NONCYTOPATHIC BOVINE VIRAL DIARRHEA VIRUS STRAIN-SD-1
DENG, RT
BROCK, KV
[J]. VIROLOGY, 1992, 191 (02) : 867 - 879
[8] DINTER Z, 1963, ZBL BAKT PARASIT, V188, P475
[9] A SIMPLE AND VERY EFFICIENT METHOD FOR GENERATING CDNA LIBRARIES
GUBLER, U
HOFFMAN, BJ
[J]. GENE, 1983, 25 (2-3) : 263 - 269
[10] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4
LAEMMLI, UK
[J]. NATURE, 1970, 227 (5259) : 680 - +

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