HPLC STUDY OF MANCOZEB DEGRADATION ON LEAVES

An HPLC procedure was developed for the determination of mancozeb on cucumber leaves after application of mancozeb suspension (ethylenebisdithiocarbamate fungicide). The analytical procedure consisted of a single extraction step with EDTA aqueous solution and sodium salt of ethylenebisdithiocarbamate was eluated from Separon NH2 column. Mobile phase was 5 % acetonitrile and 45 % methanal in aqueous solution of borate buffer at pH = 7.6 . The level of mancozeb after application on leaves decreased slowly, with a half time 14 days.