LIGHT-EMITTING PROPERTIES OF RECOMBINANT SEMISYNTHETIC AEQUORINS AND RECOMBINANT FLUORESCEIN-CONJUGATED AEQUORIN FOR MEASURING CELLULAR CALCIUM

被引：117

作者：

SHIMOMURA, O

MUSICKI, B

KISHI, Y

INOUYE, S

机构：

[1] CHISSO CORP, YOKOHAMA RES CTR, YOKOHAMA, JAPAN

[2] BOSTON UNIV, SCH MED, DEPT PHYSIOL, BOSTON, MA 02118 USA

[3] HARVARD UNIV, DEPT CHEM, CAMBRIDGE, MA 02138 USA

来源：

CELL CALCIUM | 1993年 / 14卷 / 05期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0143-4160(93)90041-4

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

15 kinds of recombinant semi-synthetic aequorins and a recombinant fluorescein-conjugated aequorin were prepared and their properties in Ca2+-triggered luminescence were studied. The semi-synthetic aequorins showed a wide range of Ca2+-sensitivity. The luminescence intensity of a high-sensitivity type (hcp-aequorin) was greater than 10(4)-times that of a low-sensitivity type (n)-aequorin) at pCa 6.0-6.5. The fluorescein-conjugated aequorin exhibited fluorescence in addition to the Ca2+-triggered luminescence, thus it can be used to visualize the diffusion and distribution of aequorin in cells. The data obtained, particularly the Ca2+-sensitivity curves, are useful in selecting a suitable semi-synthetic aequorin for an experiment

引用

页码：373 / 378

页数：6

共 21 条

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