STRINGS OF CONTIGUOUS MODIFIED PENTANUCLEOTIDES WITH INCREASED DNA-BINDING AFFINITY CAN BE USED FOR DNA-SEQUENCING BY PRIMER WALKING

被引：31

作者：

AZHIKINA, T ^{[1
]}

VESELOVSKAYA, S ^{[1
]}

MYASNIKOV, V ^{[1
]}

POTAPOV, V ^{[1
]}

ERMOLAYEVA, O ^{[1
]}

SVERDLOV, E ^{[1
]}

机构：

[1] MM SHEMYAKIN OVCHINNIKOV BIOORGAN CHEM INST,HUMAN GENOME STRUCT & FUNCT LAB,MOSCOW 117871,RUSSIA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 24期

关键词：

MODIFIED OLIGONUCLEOTIDES; PRIMERS; GENOME WALKING; SINGLE-STRANDED DNA-BINDING PROTEIN;

D O I：

10.1073/pnas.90.24.11460

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Modified oligonucleotides containing 5-methylcytidine and/or 2-aminoadenosine form tighter hybrids with DNA and are, therefore, more efficient primers for DNA sequencing as compared to their natural counterparts. Strings of contiguous modified pentanucleotides can be used for DNA sequencing by primer walking.

引用

页码：11460 / 11462

页数：3

共 11 条

[1]

AZHIKINA TL, 1993, DOKL AKAD NAUK+, V330, P642

[2] INSITU ACTIVATION OF BIS-DIALKYLAMINOPHOSPHINES - A NEW METHOD FOR SYNTHESIZING DEOXYOLIGONUCLEOTIDES ON POLYMER SUPPORTS [J].