IDENTIFICATION OF BASES IN 16S-RIBOSOMAL RNA ESSENTIAL FOR TRANSFER-RNA BINDING AT THE 30S-RIBOSOMAL P-SITE

被引：99

作者：

VONAHSEN, U ^{[1
]}

NOLLER, HF ^{[1
]}

机构：

[1] UNIV CALIF SANTA CRUZ, SINSHEIMER LABS, SANTA CRUZ, CA 95064 USA

来源：

SCIENCE | 1995年 / 267卷 / 5195期

关键词：

D O I：

10.1126/science.7528943

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Previous studies suggest that the mechanism of action of the ribosome in translation involves crucial transfer RNA (tRNA)-ribosomal RNA (rRNA) interactions. Here, a selection scheme was developed to identify bases in 16S rRNA that are essential for tRNA binding to the P site of the small (30S) ribosamal subunit. Modification of the N-1 and N-2 positions of 2-methylguanine 966 and of the N-7 position of guanine 1401 interfered with messenger RNA (mRNA)-dependent binding of tRNA to the P site. Modification of the same positions as well as of the N-1 and N-2 positions of guanine 926 interfered with mRNA-independent binding of tRNA at high magnesium ion concentration. These results suggest that these three bases are involved in intermolecular contacts between ribosomes and tRNA.

引用

页码：234 / 237

页数：4

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