CHARACTERIZATION OF NA+/H+-ANTIPORTER GENE CLOSELY-RELATED TO THE SALT-TOLERANCE OF YEAST ZYGOSACCHAROMYCES-ROUXII

被引：75

作者：

WATANABE, Y

MIWA, S

TAMAI, Y

机构：

[1] Department of Biological Resources, Faculty of Agriculture, Ehime University, Matsuyama, Ehime

来源：

YEAST | 1995年 / 11卷 / 09期

关键词：

SALT-TOLERANT YEAST; ZYGOSACCHAROMYCES ROUXII; NA+/H+-ANTIPORTER; GENE DISRUPTION; SALT TOLERANCE;

D O I：

10.1002/yea.320110905

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to clarify the relationship between salt-tolerance of Zygosaccharomyces rouxii and the function of Na+/H+-antiporter, a gene was isolated from Z. rouxii which exhibited homology to the Na+/H+-antiporter gene (sod2) from Schizosaccharomyces pombe. This newly isolated gene (Z-SOD2) encoded a product of 791 amino acids, which was larger than the product encoded by its Sz. pombe homologue. The predicted amino-acid sequence of Z-Sod2p was highly homologous to that of the Sz. pombe protein, but included an extra-hydrophilic stretch in the C-terminal region. The expression of Z-SOD2 was constitutive and independent of NaCl-shock. Z-SOD2-disruptants of Z. rouxii did not grow in media supplemented with 3 M-NaCl, but grew well in the presence of 50% sorbitol, indicating that the function of Z-SOD2 was closely related to the salt-tolerance of Z. rouxii. Several genes are also compared and discussed in relation to the salt-tolerance of Z. rouxii. The nucleotide sequence data reported in this paper will appear in the GSDB, DDBJ, EMBL and NCBI nucleotide sequence databases with the following accession number: D43629.

引用

页码：829 / 838

页数：10

共 24 条

[1]

Carlson M., Botstein D., Two differentially regulated mRNAs with different 5′ ends encode secreted and intracellular forms of yeast invertase, Cell, 28, pp. 145-154, (1982)

[2]

Eddy A.A., Mechanisms of solute transport in selected eukaryotic microorganisms, Adv. Microbiol. Physiol., 23, pp. 1-78, (1982)

[3]

Haro M., Garciadeblas B., Rodriguez-Navarro A., A novel P‐type ATPase from yeast involved in sodium transport, FEBS Lett., 291, pp. 189-191, (1991)

[4]

Jia Z.-P., McCullough N., Martel R., Hemmingsen S., Young P.G., Gene amplification at a locus encoding a putative Na+/H+ antiporter confers sodium and lithium tolerance in fission yeast, EMBO J., 11, pp. 1631-1640, (1992)

[5]

Katz A., Kaback H.R., Avron M., Na+/H+ antiport in isolated plasma membrane vesicles from the halotolerant alga Dunaliella salina, FEBS Lett., 202, pp. 141-144, (1986)

[6]

Katz A., Pick U., Avron M., Characterization and reconstitution of the Na+/H+ antiporter from the plasma membrane of the halotolerant alga Dunaliella, Biochim. Biophys. Acta, 983, pp. 9-14, (1988)

[7]

Kyte J., Doolittle R.F., A simple method for displaying the hydrophobic character of a protein, J. Mol. Biol., 157, pp. 105-132, (1982)

[8]

Onishi H., Osmophilic yeasts. IV. Changes in permeability of cell membranes of the osmophilic yeasts and maintenance of their viability in the saline medium, Bulletin of the Agricultural Chemical Society of Japan, 23, pp. 332-339, (1959)

[9]

Rodriguez-Navaro A., Ortega M.D., The mechanism of sodium efflux in yeast, FEBS Lett., 138, pp. 205-208, (1982)

[10]

Rose M.D., Winston F., Hieter P., Methods in Yeast Genetics: A Laboratory Course Manual, (1990)

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