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ANALYTICAL APPLICATION OF MICROCALORIMETRY - A STOP-FLOW MIXING DEVICE

被引：8
作者：
PENNINGTON, SN
BROWN, HD
BERGER, RL
EVANS, WJ
机构：
[1] Biochemistry Section, Cancer Research Center, Columbia
[2] Laboratory for Instrumental Development, National Heart Institute, Bethesda
[3] Southern Regional Research Laboratory, Department of Agriculture, New Orleans, LA
来源：
ANALYTICAL BIOCHEMISTRY | 1969年 / 32卷 / 02期
关键词：
D O I：
10.1016/0003-2697(69)90082-7
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A stopped-flow (stop-start) mixing device for use in connection with a bench-top microcalorimeter has been designed and constructed. The device employs a ball mixer for rapid combination of solutions pre-equilibrated in spirals of tantalum tubing. Following the mixing procedure, the resultant heat is measured by pumping the mixed solution into Teflon coils situated within the sensing volume of a microcalorimeter. The sensitivity of the flow mixer is comparable to other types of mixers employed in the same instrument and allows serial analyses once equilibrium has been obtained. The sealed nature of the flow cells allows reactions that are sensitive to their environment to be carried out without resorting to inert atmospheres. © 1969.
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页码：251 / +
页数：1
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