SATURATION OF DNA-REPAIR IN MAMMALIAN-CELLS

被引：28

作者：

AHMED, FE

SETLOW, RB

机构：

[1] Department of Biology, Brookhaven National Laboratory, Upton, New York

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1979年 / 29卷 / 05期

关键词：

D O I：

10.1111/j.1751-1097.1979.tb07802.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract—Excision repair seems to reach a plateau in normal human cells at a 254 nm dose near 20J/m2. We measured excision repair in normal human fibroblasts up to 80J/m2. The four techniques used (unscheduled DNA synthesis, photolysis of BrdUrd incorporated during repair, loss of sites sensitive to a UV endonuclease from Micrococcus luteus, and loss of pyrimidine dimers from DNA) showed little difference between the two doses. Moreover, the loss of endonuclease sites in 24 h following two 20J/m2 doses separated by 24 h was similar to the loss observed following one dose. Hence, we concluded that the observed plateau in excision repair is real and does not represent some inhibitory process at high doses but a true saturation of one of the, rate limiting steps in repair. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：983 / 989

页数：7

共 34 条

[1]

AHMED FE, 1977, CANCER RES, V37, P3414

[2] EXCISION REPAIR IN ATAXIA TELANGIECTASIA, FANCONIS ANEMIA, COCKAYNE SYNDROME, AND BLOOMS SYNDROME AFTER TREATMENT WITH ULTRAVIOLET-RADIATION AND N-ACETOXY-2-ACETYLAMINOFLUORENE [J].