ACTIVATION OF FROG EPIDERMIS TYROSINASE BY IMMOBILIZATION ON A SOLID SUPPORT

被引：10

作者：

IBORRA, JL

MANJON, A

TARI, M

机构：

[1] Departamento de Bioquímica, Facultad de Ciencias y Colegio de Medicina, Alicante

来源：

GENERAL PHARMACOLOGY-THE VASCULAR SYSTEM | 1979年 / 10卷 / 02期

关键词：

D O I：

10.1016/0306-3623(79)90049-1

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

1. Frog epidermis protyrosinase was activated by immobilization on Enzacryl AA and CNBractivated Sepharose. 2. The proenzyme immobilized through its tyrosine and lysine residues reached 28% and 19% of soluble enzyme activity, respectively. 3. Fluorescence spectra (ιem), half-inactivation times (t 1 2), pH-activity profile and apparent Michaelis constant (K′m) of immobilized protein were compared with that of the soluble enzyme. 4. The data obtained supported our previous hypothesis that activation mechanism of proenzyme involves a more unfolded conformational state. © 1979.

引用

页码：143 / 145

页数：3

共 23 条

[1] ACTIVATION OF PRE-PHENOL OXIDASE IN HEMOLYMPH OF SILKWORM BOMBYX MORI [J].