DIRECT AMPLIFICATION OF DNA FROM COLONIES OF BACILLUS-SUBTILIS AND ESCHERICHIA-COLI BY THE POLYMERASE CHAIN-REACTION

被引：42

作者：

SARIS, PEJ ^{[1
]}

PAULIN, LG ^{[1
]}

UHLEN, M ^{[1
]}

机构：

[1] ROYAL INST TECHNOL,DEPT BIOCHEM & BIOTECHNOL,S-10044 STOCKHOLM 70,SWEDEN

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1990年 / 11卷 / 02期

关键词：

D O I：

10.1016/0167-7012(90)90012-U

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The polymerase chain reaction (PCR) catalyzed by Taq DNA polymerase has been used to amplify genomic and plasmid DNA sequences. In all reported cases, the template has been extensively purified. We show here that genomic and plasmid DNA sequences can be amplified by the PCR directly from single colonies of both Escherichia coli and Bacillus subtilis without any purification of the template. This technique makes the use of the PCR even more applicable in the automation processes, screening and diagnosis. © 1990.

引用

页码：121 / 126

页数：6

共 18 条

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