NEW METHOD FOR DETECTING RHAMNOLIPIDS EXCRETED BY PSEUDOMONAS SPECIES DURING GROWTH ON MINERAL AGAR

被引：340

作者：

SIEGMUND, I

WAGNER, F

机构：

[1] Institute of Biochemistry and Biotechnology, Technical University of Braunschweig, Braunschweig, D-3300

来源：

BIOTECHNOLOGY TECHNIQUES | 1991年 / 5卷 / 04期

关键词：

D O I：

10.1007/BF02438660

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new semi-quantitative agar plate test for the detection of extracellular rhamnolipids has been developed. These biological anionic tensides (biosurfactants) form an insoluble ion pair with the cationic tenside cetyltrimethylammonium bromide and the basic dye methylene blue which was included in mineral agar plates. On the light blue agar, productive colonies of Pseudomonas spec. were surrounded by dark blue halos. The test is specific for anionic biosurfactants and can be applied to other glycolipid producing microorganisms.

引用

页码：265 / 268

页数：4

共 10 条

[1] MICROBIAL-PRODUCTION, STRUCTURE ELUCIDATION AND BIOCONVERSION OF SOPHOROSE LIPIDS [J].