COLORIMETRIC DETERMINATION OF GALACTOSE AND GALACTOSE-1-PHOSPHATE FROM DRIED BLOOD

被引：36

作者：

DIEPENBROCK, F ^{[1
]}

HECKLER, R ^{[1
]}

SCHICKLING, H ^{[1
]}

ENGELHARD, T ^{[1
]}

BOCK, D ^{[1
]}

SANDER, J ^{[1
]}

机构：

[1] STAATLICHES MED ALUNTERSUCHUNGSAMT,ROESEBECKSTR 4-6,W-3000 HANNOVER 91,GERMANY

来源：

CLINICAL BIOCHEMISTRY | 1992年 / 25卷 / 01期

关键词：

GALACTOSEMIA; COLORIMETRY; NEWBORN SCREENING;

D O I：

10.1016/0009-9120(92)80043-G

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A colorimetric microassay for the simultaneous quantitative determination of galactose (Gal) and galactose-1-phosphate (Gal-1-P) in dried blood spots is described. An enzymatic reaction involving alkaline phosphatase (EC 3.1.3.1) and galactose dehydrogenase (EC 1.1.1.48) produces NADH, which is coupled with diaphorase (EC 1.8.1.4) and iodonitrotetrazolium violet (INT). The colourless INT is converted to a formazan of red colour the intensity of which is quantitated either photometrically by a microplate reader or determined visually with sufficient sensitivity for screening purposes. We evaluated the assay on 200,000 blood samples in a newborn screening program, and were able to distinguish between classical and milder forms of galactosemia with ease.

引用

页码：37 / 39

页数：3

共 4 条

[1] SIMULTANEOUS QUANTITATIVE ESTIMATION OF GALACTOSE-1-PHOSPHATE AND GALACTOSE IN BLOOD FOR THE DIAGNOSIS OF GALACTOSEMIA [J].

FUJIMURA, Y ;

KAWAMURA, M ;

NARUSE, H .

TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE, 1982, 137 (03) :289-295

[2] MICRO-DETERMINATION OF GALACTOSE AND GALACTOSE 1-PHOSPHATE IN DRIED BLOOD SPOTS [J].

FUJIMURA, Y ;

ISHII, S ;

KAWAMURA, M ;

NARUSE, H .

ANALYTICAL BIOCHEMISTRY, 1981, 117 (01) :187-195

[3] MICROASSAY FOR ESTIMATION OF GALACTOSE AND GALACTOSE-1-PHOSPHATE IN DRIED BLOOD SPECIMENS [J].

ORFANOS, AP ;

JINKS, DC ;

GUTHRIE, R .

CLINICAL BIOCHEMISTRY, 1986, 19 (04) :225-228

[4]

YAMAGUCHI A, 1989, CLIN CHEM, V35, P1962

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