EFFECTS OF BILIRUBIN ON POTASSIUM (RB-86(+)) INFLUX AND IONIC CONTENT IN EHRLICH ASCITES-CELLS

被引：10

作者：

CORCHS, JL ^{[1
]}

SERRANI, RE ^{[1
]}

PALCHICK, M ^{[1
]}

机构：

[1] HOSP PROVINCIAL ROSARIO,CTR MED NUCL,ROSARIO 2000,ARGENTINA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 555卷 / 03期

关键词：

(Ehrlich ascites cell); !sup]86[!/sup]Rb[!sup]+[!/sup; Bilirubin; Intracellular K[!sup]+[!/sup; Intracellular Na[!sup]+[!/sup; K[!sup]+[!/sup] influx;

D O I：

10.1016/0005-2736(79)90404-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Potassium influx, intracellular potassium and sodium content and cellular volume were determined in vitro in Ehrlich ascites cells in the presence of up to 0.8 mM bilirubin in the incubation medium. Bilirubin uptake into cells as a function of bilirubin concentration in the incubation medium increased linearly with a molar bilirubin/albumin ratio of 20 : 1. Potassium influx and intracellular content decreased while cellular volume increased after 180 min of incubation of cells in bilirubin at a molar bilirubin/albumin ratio of 20 : 1. At a bilirubin/albumin ratio 2 : 1, potassium influx decreased, cellular volume remained unchanged, and bilirubin uptake into cells became saturated at bilirubin concentrations greater than 0.3 mM. It is suggested that bilirubin-induced alterations in potassium gradients across cell membranes may play a role in toxic effects of bilirubin on cells. © 1979.

引用

页码：512 / 518

页数：7

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