LOW-STRINGENCY PCR WITH DIAGNOSTICALLY USEFUL PRIMERS FOR IDENTIFICATION OF LEPTOSPIRA SEROVARS

被引：13

作者：

DECABALLERO, OLSD

NETO, ED

KOURY, MC

ROMANHA, AJ

SIMPSON, AJG

机构：

[1] FIOCRUZ MS,CTR PESQUISAS RENE RACHOU,BR-30190 BELO HORIZONT,MG,BRAZIL

[2] FAC MED TRIANGULO MINEIRO,DEPT CIENCIAS BIOL,BR-38015 UBERABA,MG,BRAZIL

[3] UNIV FED MINAS GERAIS,INST CIENCIAS BIOL,DEPT MICROBIOL,BR-31270 BELO HORIZONT,MG,BRAZIL

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1994年 / 32卷 / 05期

关键词：

D O I：

10.1128/JCM.32.5.1369-1372.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Primers proposed for the diagnosis of the pathogenic spirochete Leptospira spp. (C. Gravekamp, H. V. D. Kemp, M, Franzen, D. Carrington, G. J. Schoone, G. J. J. M Van Eys, C. O. R. Everard, R. A. Hartskeel, and W. J. Terpstra, J. Gen. Microbiol. 139:1691-1700, 1993) have been found to produce complex serovar-specific patterns under low-stringency PCR conditions. Such patterns obtained by low-stringency PCR, which maintain the specific band as an internal control, offer an approach to the standardized identification of Leptospira serovars in clinical laboratories.

引用

页码：1369 / 1372

页数：4

共 14 条

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FAINE S, 1982, WHO OFFSET PUBLICATI

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