FLUORESCENCE LIFETIME-BASED SENSING IN TISSUES - A COMPUTATIONAL STUDY

被引：77

作者：

HUTCHINSON, CL

LAKOWICZ, JR

SEVICKMURACA, EM

机构：

[1] PURDUE UNIV,SCH CHEM ENGN,W LAFAYETTE,IN 47907

[2] UNIV MARYLAND,DEPT BIOL CHEM,CTR FLUORESCENCE SPECT,BALTIMORE,MD 21201

来源：

BIOPHYSICAL JOURNAL | 1995年 / 68卷 / 04期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

D O I：

10.1016/S0006-3495(95)80330-9

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We have numerically solved the photon diffusion equation to predict the distribution of light ina tissue model system with a uniform concentration of fluorophore. Our results show that time-dependent measurements of light propagation can be used to monitor the fluorescent lifetimes of a uniformly distributed fluorophore in tissues. With proper referencing, frequency-domain measurements of phase-shift, theta, may allow quantitation of fluorescent lifetimes, tau, independent of changes in the local absorption nd scattering properties. These results point to a new approach for noninvasive diagnostic monitoring through quantitation of fluorescent lifetime, tau, when the lifetime of the fluorophore is comparable with photon migration times.

引用

页码：1574 / 1582

页数：9

共 28 条

[1] THE THEORETICAL BASIS FOR THE DETERMINATION OF OPTICAL PATHLENGTHS IN TISSUE - TEMPORAL AND FREQUENCY-ANALYSIS
ARRIDGE, SR
COPE, M
DELPY, DT
[J]. PHYSICS IN MEDICINE AND BIOLOGY, 1992, 37 (07) : 1531 - 1560
[2] DETERMINATION OF OXYGEN CONCENTRATIONS BY LUMINESCENCE QUENCHING OF A POLYMER-IMMOBILIZED TRANSITION-METAL COMPLEX
BACON, JR
DEMAS, JN
[J]. ANALYTICAL CHEMISTRY, 1987, 59 (23) : 2780 - 2785
[3] BURCH CL, 1994, SPIE, V2135, P286
[4] PHOTOPHYSICS AND PHOTOCHEMISTRY OF OXYGEN SENSORS BASED ON LUMINESCENT TRANSITION-METAL COMPLEXES
CARRAWAY, ER
DEMAS, JN
DEGRAFF, BA
BACON, JR
[J]. ANALYTICAL CHEMISTRY, 1991, 63 (04) : 337 - 342
[5] DRAXLER S, 1993, SENSOR ACTUAT B-CHEM, V11, P421, DOI 10.1016/0925-4005(93)85282-F
[6] RELATION BETWEEN FLUORESCENCE-SPECTRA OF DILUTE AND TURBID SAMPLES
DURKIN, AJ
JAIKUMAR, S
RAMANUJAM, N
RICHARDSKORTUM, R
[J]. APPLIED OPTICS, 1994, 33 (03): : 414 - 423
[7] HUTCHINSON CL, 1995, IN PRESS SPIE
[8] FLUORESCENCE SPECTROSCOPY OF TURBID MEDIA - AUTOFLUORESCENCE OF THE HUMAN AORTA
KEIJZER, M
RICHARDSKORTUM, RR
JACQUES, SL
FELD, MS
[J]. APPLIED OPTICS, 1989, 28 (20): : 4286 - 4292
[9] Lakowicz J. R., 1983, PRINCIPLES FLUORESCE
[10] FLUORESCENCE LIFETIME-BASED SENSING OF PH, CA-2(+), K+ AND GLUCOSE
LAKOWICZ, JR
SZMACINSKI, H
[J]. SENSORS AND ACTUATORS B-CHEMICAL, 1993, 11 (1-3) : 133 - 143

← 1 2 3 →