SECRETION OF CELLULASE AND BETA-GLUCOSIDASE BY TRICHODERMA-VIRIDE ITCC-1433 IN SUBMERGED CULTURE ON DIFFERENT SUBSTRATES

Trichoderma viride ITCC‐1433 produces high yields of cellulase and especially β‐glucosidase when grown in submerged culture on different carbon sources. Cellulase synthesis was strongly repressed in the presence of glucose and only a low constitutive activity of β‐glucosidase and carboxymethylcellulase, but no Avicelase, could be demonstrated when culturing T. viride on glucose. With carboxymethylcellulose (CMC) as a substrate the secretion of enzyme as well as growth depended on the degree of substitution, but in general CMC cannot be regarded either as a powerful inducer or as a carbon source. With insoluble cellulose, maximum enzyme production and activities were obtained using an alkali‐treated cellulose powder. On this substrate the excretion of soluble protein into the culture broth increased and the protein concentration corresponded to cellulolytic activities. Copyright © 1979 John Wiley & Sons, Inc.