SEPARATION OF ALGAL MIXTURES AND BACTERIAL MIXTURES WITH FLOW-MICROFLUOROMETER USING CHLOROPHYLL AND ETHIDIUM-BROMIDE FLUORESCENCE

被引：22

作者：

PAAU, AS

COWLES, JR

ORO, J

BARTEL, A

HUNGERFORD, E

机构：

[1] UNIV HOUSTON,DEPT BIOL,HOUSTON,TX 77004

[2] UNIV HOUSTON,DEPT PHYS,HOUSTON,TX 77004

[3] UNIV HOUSTON,DEPT BIOPHYS SCI,HOUSTON,TX 77004

来源：

ARCHIVES OF MICROBIOLOGY | 1979年 / 120卷 / 03期

关键词：

Chlorophyll fluorescence; Ethidium bromide; Flow-microfluorometry; Microbial cells; Nucleic acid; Separation;

D O I：

10.1007/BF00423075

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The applicability of flow-microfluorometer to separate microbial cells was demonstrated with algal and bacterial cells. Algal mixtures were sorted according to the natural chlorophyll fluorescence and the bacterial mixtures were sorted according to the fluorescence of ethidium bromide-stained nucleic acid. © 1979 Springer-Verlag.

引用

页码：271 / 273

页数：3

共 7 条

[1] CHARACTERIZATION OF BACTERIAL-GROWTH BY MEANS OF FLOW MICROFLUOROMETRY
BAILEY, JE
FAZELMADJLESSI, J
MCQUITTY, DN
LEE, LY
ALLRED, JC
ORO, JA
[J]. SCIENCE, 1977, 198 (4322) : 1175 - 1176
[2] CHROMOSOME MEASUREMENT AND SORTING BY FLOW SYSTEMS
GRAY, JW
CARRANO, AV
STEINMETZ, LL
VANDILLA, MA
MOORE, DH
MAYALL, BH
MENDELSOHN, ML
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (04) : 1231 - 1234
[3] QUANTITATIVE SINGLE CELL ANALYSIS AND SORTING
HORAN, PK
WHEELESS, LL
[J]. SCIENCE, 1977, 198 (4313) : 149 - 157
[4] COMPARISON OF NUCLEIC-ACID CONTENT IN POPULATIONS OF FREE-LIVING AND SYMBIOTIC RHIZOBIUM-MELILOTI BY FLOW MICROFLUOROMETRY
PAAU, AS
LEE, D
COWLES, JR
[J]. JOURNAL OF BACTERIOLOGY, 1977, 129 (02) : 1156 - 1158
[5] FLOW-MICROFLUOROMETRIC ANALYSIS OF ESCHERICHIA-COLI, RHIZOBIUM-MELILOTI, AND RHIZOBIUM-JAPONICUM AT DIFFERENT STAGES OF GROWTH-CYCLE
PAAU, AS
COWLES, JR
ORO, J
[J]. CANADIAN JOURNAL OF MICROBIOLOGY, 1977, 23 (09) : 1165 - 1169
[6] APPLICATION OF FLOW MICRO-FLUOROMETRY TO STUDY OF ALGAL CELLS AND ISOLATED-CHLOROPLASTS
PAAU, AS
ORO, J
COWLES, JR
[J]. JOURNAL OF EXPERIMENTAL BOTANY, 1978, 29 (111) : 1011 - 1020
[7] STEINKAMP JA, 1973, REV SCI INSTRUM, V44, P1301, DOI [10.1063/1.1686375, 10.1063/1.1686082]

← 1 →