MUTANT OF THE GLUTAMINE TRANSFER-RNA GENE AS UGA SUPPRESSOR IN ESCHERICHIA-COLI

被引：4

作者：

INOKUCHI, H

KONDO, K

YOSHIMURA, M

OZEKI, H

机构：

[1] Department of Biophysics, Faculty of Science, Kyoto University, Sakyo-ku, Kyoto

来源：

MOLECULAR AND GENERAL GENETICS | 1990年 / 223卷 / 03期

关键词：

Escherichia coli; Lethal effect; tRNA[!sub]2[!/sub][!sup]Gln[!/sup]gene; UGA suppressor;

D O I：

10.1007/BF00264450

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A UGA suppressor derived from a glutamine tRNA gene of Escherichia coli K 12 was isolated and characterized. Phages carrying the suppressor su+2UGA could be obtained only from a hybrid transducing phage, h80cI857p su+2oc, but not from the original transducing phage λcI857p su+2oc. By DNA sequence analysis, it was found that the su+2 UGA suppressor obtained has two mutations; one is in the anticodon (TTA→TCA), as expected, and the other (C→T) is at the 7th position from the 3′ end of tRNA2Gln. The significance of these mutations and the lethal effect on phage λ of the increased amounts of UGA suppressor tRNAs are discussed. © 1990 Springer-Verlag.

引用

页码：433 / 437

页数：5

共 16 条

[1] OPTIMAL CONDITIONS FOR MUTAGENESIS BY N-METHYL-N'-NITRO-N-NITROSOGUANIDINE IN ESCHERICHIA COLI K12 [J].