STRUCTURE AND EXPRESSION OF A TOBACCO BETA-1,3-GLUCANASE GENE

被引：105

作者：

OHMETAKAGI, M ^{[1
]}

SHINSHI, H ^{[1
]}

机构：

[1] FERMENTAT RES INST,PLANT MOLEC BIOL LAB,TSUKUBA,IBARAKI 305,JAPAN

来源：

PLANT MOLECULAR BIOLOGY | 1990年 / 15卷 / 06期

关键词：

BETA-1,3-GLUCANASE GENE; ETHYLENE; GENE FAMILY; TOBACCO;

D O I：

10.1007/BF00039434

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We determined the primary structure of a tobacco-beta-1,3-glucanase gene. The beta-1,3-glucanase gene has a single large intron, and the intron separates coding regions of the signal peptide and the mature enzyme. Analysis of the 5'-flanking region sequence revealed an 11 bp GC-rich element with perfect homology to the putative regulatory sequence of tobacco chitinase genes. RNA blot analysis showed that levels of mRNAs of beta-1,3-glucanase and chitinase are coordinately increased in response to ethylene and salicylic acid. Accumulation of beta-1,3-glucanase mRNA in suspension-cultured cells is rapidly induced at late logarithmic growth phase. Members of the tobacco-beta-1,3-glucanase gene families are classified into two subfamilies. One of the subfamilies appeared to be transcriptionally inactive.

引用

页码：941 / 946

页数：6

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