ATRIAL-NATRIURETIC-PEPTIDE BINDS TO ANP-R(1) RECEPTORS IN NEUROBLASTOMA-CELLS OR IS DEGRADED EXTRACELLULARLY AT THE SER-PHE BOND

被引：23

作者：

DELPORTE, C ^{[1
]}

POLOCZEK, P ^{[1
]}

TASTENOY, M ^{[1
]}

WINAND, J ^{[1
]}

CHRISTOPHE, J ^{[1
]}

机构：

[1] UNIV LIBRE BRUXELLES,SCH MED,DEPT BIOCHEM & NUTR,BLDG G-E,CP 611,ROUTE LENNIK 808,B-1070 BRUSSELS,BELGIUM

来源：

EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION | 1992年 / 227卷 / 03期

关键词：

ANP-R(1) RECEPTORS; CGMP; NEUTRAL METALLOENDOPEPTIDASE; HUMAN NEUROBLASTOMA CELL LINE NB-OK1;

D O I：

10.1016/0922-4106(92)90002-D

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

ANP-R1 receptors for atrial natriuretic peptide (ANP) showed the following rank order of affinity in intact human neuroblastoma cells NB-OK-1: human ANP-(99-126) almost-equal-to human ANP-(102-126) almost-equal-to rat ANP-(99-126) (K(I) 17-32 pM) > human ANP-(103-126) > porcine brain natriuretic peptide (BNP). Analogues truncated at the C-terminal extremity or devoid of a disulphide bridge, such as rat ANP-(103-123), rat C-ANP-(102-121), rat ANP-(111-126), rat ANP-(99-109) and rat [desCys105,Cys121]ANP-(104-126) and chicken C-type natriuretic peptide, were not recognized. The occupancy of these high affinity ANP-R1 receptors led to marked cyclic GMP accumulation in the presence of 3-isobutyl 1-methylxanthine. An ectoenzymic activity, partly shed in the incubation medium, provoked the stepwise release of Phe-Arg-[I-125]Tyr, Arg-[I-125]Tyr and [I-125]Tyr from rat [I-125]ANP-(99-126), at an optimal pH of 7.0. Its inhibition by 1,10-phenanthroline, EDTA and bacitracin but not by thiorphan suggests the contribution of at least one neutral metalloendopeptidase, distinct from EC 3.4.24.11, for which ANP showed high affinity.

引用

页码：247 / 256

页数：10

共 50 条

[1]

ATHERTON E, 1985, J CHEM SOC CHEM COMM, V3, P165

[2]

Bergmeyer HU, 1974, METHOD ENZYMAT AN, P574

[3] THE HYDROLYSIS OF BRAIN AND ATRIAL NATRIURETIC PEPTIDES BY PORCINE CHOROID-PLEXUS IS ATTRIBUTABLE TO ENDOPEPTIDASE-24.11 [J].

BOURNE, A ;

KENNY, AJ .

BIOCHEMICAL JOURNAL, 1990, 271 (02) :381-385

[4] DISTRIBUTION OF ATRIAL-NATRIURETIC-PEPTIDE RECEPTOR SUBTYPES IN RAT-BRAIN [J].

BROWN, J ;

CZARNECKI, A .

AMERICAN JOURNAL OF PHYSIOLOGY, 1990, 258 (04) :R1078-R1083

[5] A PEPTIDE-HORMONE-INACTIVATING ENDOPEPTIDASE IN XENOPUS-LAEVIS SKIN SECRETION [J].

CARVALHO, KD ;

JOUDIOU, C ;

BOUSSETTA, H ;

LESENEY, AM ;

COHEN, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (01) :84-88

[6] DIFFERENTIAL ACTIVATION BY ATRIAL AND BRAIN NATRIURETIC PEPTIDES OF 2 DIFFERENT RECEPTOR GUANYLATE CYCLASES [J].

CHANG, M ;

LOWE, DG ;

LEWIS, M ;

HELLMISS, R ;

CHEN, E ;

GOEDDEL, DV .

NATURE, 1989, 341 (6237) :68-72

[7]

CHENG Y, 1973, BIOCHEM PHARMACOL, V22, P3099

[8] A MEMBRANE FORM OF GUANYLATE-CYCLASE IS AN ATRIAL NATRIURETIC PEPTIDE RECEPTOR [J].

CHINKERS, M ;

GARBERS, DL ;

CHANG, MS ;

LOWE, DG ;

CHIN, HM ;

GOEDDEL, DV ;

SCHULZ, S .

NATURE, 1989, 338 (6210) :78-83

[9] CHARACTERIZATION AND REGULATION OF ATRIAL-NATRIURETIC-PEPTIDE (ANP)-R1 RECEPTORS IN THE HUMAN NEUROBLASTOMA CELL-LINE NB-OK-1 [J].

DELPORTE, C ;

POLOCZEK, P ;

GOSSEN, D ;

TASTENOY, M ;

WINAND, J ;

CHRISTOPHE, J .

EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION, 1991, 207 (01) :81-88

[10] A NEW METALLOENDOPEPTIDASE FROM HUMAN NEUROBLASTOMA NB-OK-1 CELLS WHICH INACTIVATES ATRIAL-NATRIURETIC-PEPTIDE BY SELECTIVE CLEAVAGE AT THE SER123-PHE124 BOND [J].

DELPORTE, C ;

CARVALHO, KD ;

LESENEY, AM ;

WINAND, J ;

CHRISTOPHE, J ;

COHEN, P .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 182 (01) :158-164

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