HUMAN PREIMPLANTATION EMBRYO CRYOPRESERVATION - SELECTED ASPECTS

被引：25

作者：

FUGGER, EF ^{[1
]}

BUSTILLO, M ^{[1
]}

DORFMANN, AD ^{[1
]}

SCHULMAN, JD ^{[1
]}

机构：

[1] GENET & IVF INST,FAIRFAX,VA 22031

来源：

HUMAN REPRODUCTION | 1991年 / 6卷 / 01期

关键词：

CRYOPRESERVATION; FROZEN-THAWED EMBRYO; PREIMPLANTION EMBRYO; ZYGOTE;

D O I：

10.1093/oxfordjournals.humrep.a137246

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

This report describes the results of cryopreserving human preimplantation zygotes and cleaved embryos (2-4 cells) in our in-vitro fertilization programme. Cryopreserved zygotes and cleaved embryos resulted in similar post-thaw survival rates (74.8 versus 70.9%). Pregnancy rates per retrieval cycle (RC) and embryos transferred per pregnancy for frozen-thawed zygotes versus frozen-thawed cleaved embryos were 21.8 versus 11.5% (P < 0.2) and 12.6 versus 17.5 (P < 0.2), respectively. Pregnancy rates increased significantly for both fresh (P < 0.0005) and frozen-thawed (P < 0.05) embryos as the number of embryos replaced per transfer increased from one to three or more. Frozen-thawed embryos resulted in multiple implantation rates per transfer of 25 compared to 6.4% (P < 0.1) for fresh embryos when two embryos were replaced. Pregnancy rates were reduced for fresh (P < 0.05) and frozen-thawed (P < 0.1) embryos obtained from patient retrieval cycle numbers > 3. The method of follicular stimulation during the retrieval cycle did not affect frozen-thawed embryo survival rates. There was no difference in pregnancy rates from frozen-thawed embryos replaced during natural or clomiphene citrate transfer cycles. Patients with cryopreserved embryos had cumulative pregnancy rates of 37.1% (66/178) compared to 23.5% (110/468) (P < 0.01) for patients with no embryos cryopreserved; cryopreservation of preimplantation embryos is a reliable therapeutic procedure that enhances achievement of pregnancy through in-vitro fertilization.

引用

页码：131 / 135

页数：5

共 10 条

[1]

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