WOUNDING NICOTIANA-TABACUM LEAVES CAUSES A DECLINE IN ENDOGENOUS INDOLE-3-ACETIC-ACID

被引：50

作者：

THORNBURG, RW

LI, XY

机构：

[1] Department of Biochemistry, Iowa State University, Ames

[2] ISU Plant Hormone Analysis Facility, 411 Bessey Hall, Iowa State University, Ames

来源：

PLANT PHYSIOLOGY | 1991年 / 96卷 / 03期

关键词：

D O I：

10.1104/pp.96.3.802

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We have previously observed that auxin can act as a repressor of the wound-inducible activation of a chimeric potato proteinase inhibitor II-CAT chimeric gene (pin2-CAT) in transgenic tobacco (Nicotiana tobacum) callus and in whole plants. Therefore, this study was designed to examine endogenous levels of indole-3-acetic acid (IAA) in plant tissues both before and after wounding. Endogenous IAA was measured in whole plant tissues by gas chromatography-mass spectrometry using an isotope dilution technique. C-13-Labeled IAA was used as an internal standard. The endogenous levels of IAA declined two- to threefold within 6 hours after a wound. The kinetics of auxin decline are consistent with the kinetics of activation of the pin2-CAT construction in the foliage of transgenic tobacco.

引用

页码：802 / 805

页数：4

共 29 条

[1] AUXIN-SYNTHESIS INHIBITION BY ABSCISIC-ACID, AND ITS REVERSAL BY GIBBERELLIC-ACID [J].