A RAPID AND RELIABLE PROCEDURE FOR EXTRACTION OF CELLULAR POLYAMINES AND INORGANIC-IONS FROM PLANT-TISSUES

被引：69

作者：

MINOCHA, R ^{[1
]}

SHORTLE, WC ^{[1
]}

LONG, SL ^{[1
]}

MINOCHA, SC ^{[1
]}

机构：

[1] UNIV NEW HAMPSHIRE,DEPT PLANT BIOL,DURHAM,NH 03824

来源：

JOURNAL OF PLANT GROWTH REGULATION | 1994年 / 13卷 / 04期

关键词：

D O I：

10.1007/BF00226036

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A fast and reliable method for the extraction of cellular polyamines and major inorganic ions (Ca, Mg, Mn, K, and P) from several plant tissues is described. The method involves repeated freezing and thawing of samples instead of homogenization. The efficiency of extraction of both the polyamines and inorganic ions by these two methods was compared for 10 different tissues. In each case, the freeze-thaw procedure resulted in a precise and quantitatively equal, or greater, yield than homogenization. Freeze-thawing not only eliminates the need for various tissue homogenizers (such as polytrons, tissumizers, and mortars and pestles), but it is so simple that a large number of samples can be processed simultaneously. We routinely processed 50-80 samples for quantitation of polyamines and inorganic ions. Freeze-thawing was equally useful for the extraction of polyamines from liver, spleen, and kidney tissues of mice.

引用

页码：187 / 193

页数：7

共 28 条

[1] SEALED CHAMBER DIGESTION FOR PLANT NUTRIENT ANALYSES [J].