DEVELOPMENT AND EVALUATION OF A MIXED-ANTIGEN ELISA FOR SERODIAGNOSIS OF ACTINOBACILLUS-PLEUROPNEUMONIAE SEROTYPES 1, 5, AND 7 INFECTIONS IN COMMERCIAL SWINE HERDS

被引：18

作者：

BOSSE, JT

FRIENDSHIP, R

ROSENDAL, S

FENWICK, BW

机构：

[1] Department of Veterinary Microbiology and Immunology, University of Guelph, Guelph

[2] Department of Population Medicine, University of Guelph, Guelph

[3] Department of Pathology and Microbiology, Kansas State University, Manhattan

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 1993年 / 5卷 / 03期

关键词：

D O I：

10.1177/104063879300500309

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A mixed-antigen enzyme-linked immunosorbent assay (ELISA) containing serotype-specific polysaccharide antigens from serotypes 1, 5, and 7 of Actinobacillus pleuropneumoniae was developed. This ELISA was evaluated using sera from experimentally infected pigs. With a negative cutoff value of 0.250 (optical density at 405 nm), sensitivity and specificity were determined to be 96% and 99.5%, respectively. The ELISA was further evaluated using sera from commercial swine. Sows and suckling piglets were found to be the best age groups for detection of positive reactors to A. pleuropneumoniae. The mixed-antigen ELISA could be used in a herd health monitoring program for detection of A. pleuropneumoniae infections.

引用

页码：359 / 362

页数：4

共 17 条

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