CMV-DNA DETECTION IN PARENCHYMATOUS ORGANS IN CASES OF SIDS

被引：15

作者：

CECCHI, R

BAJANOWSKI, T

KAHL, B

WIEGAND, P

机构：

[1] UNIV MUNSTER,INST MED LEGALE,D-48149 MUNSTER,GERMANY

[2] UNIV MUNSTER,INST MICROBIOL,D-48149 MUNSTER,GERMANY

[3] UNIV ROME,INST MED LEGALE,I-00161 ROME,ITALY

来源：

INTERNATIONAL JOURNAL OF LEGAL MEDICINE | 1995年 / 107卷 / 06期

关键词：

CMV INCLUSION DISEASE; LUNG TISSUE; PCR; NESTED PCR;

D O I：

10.1007/BF01246875

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

A nested PCR approach has been developed especially for the detection of small amounts of cytomegalovirus (CMV) DNA in autopsy samples. Lung tissue and submandibular glands in 118 cases of infant death (92 SIDS cases, 13 natural deaths due to other defined causes and 13 unnatural deaths) were investigated by this technique and compared to the results obtained by other CMV detection methods (histology, immunohistochemistry, in situ hybridization and PCR). CMV-DNA could be detected in the lung tissue in 7 cases of SIDS using nested PCR. Compared to conventional PCR (3 positive cases in lung tissue) the nested approach always gave glear results and showed less additional bands. In all cases where CMV could be detected in the lungs, positive results were also obtained in the submandibular glands. The nested PCR method proved to be a more sensitive technique than the other detection methods including PCR and hot start, and even minimal amounts of target DNA could be detected in the presence of human and bacterial background DNA.

引用

页码：291 / 295

页数：5

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