ARYLAMINE OXIDATIONS BY CHLOROPEROXIDASE

Chloroperoxidase was found to catalyze the peroxide oxidation of most of the anilines investigated to the corresponding nitroso compounds. Michaelis-Menten constants and maximal velocities were determined for each substrate at the optimal hydrogen peroxide concentration. The major factor controlling the rate of oxidation of the aniline substrates was found to be substituent size, with highly bulky substituents resulting in a marked decrease in the rate of oxidation. Turnover numbers for oxidizable substrates ranged from 67 200 min-1 for aniline down to 6800 min-1 for 4-isopropylaniline. © 1979.