CHARACTERISTICS OF THE DNA FROM LYMANTRIA-DISPAR NUCLEAR POLYHEDROSIS-VIRUS

被引：16

作者：

MCCARTHY, WJ ^{[1
]}

MURPHY, TF ^{[1
]}

LANGRIDGE, W ^{[1
]}

机构：

[1] PENN STATE UNIV, CTR GRAD STUDY, UNIVERSITY PK, PA 16802 USA

来源：

VIROLOGY | 1979年 / 95卷 / 02期

关键词：

D O I：

10.1016/0042-6822(79)90514-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The DNA isolated from occluded Lymantria dispar nuclear polyhedrosis virus consisted of double-stranded linear, open circular, and covalently closed molecules. The DNA had a guanosine plus cytosine content (G + C) of 59-62% as estimated from both thermal denaturation and CsCl density gradient centrifugation. The L. dispar host DNA had a G + C content of 38-39%. Comparisons of molecular weight estimations for viral DNA by sedimentation on neutral sucrose gradients, electron microscopy, and reassociation kinetics gave values of 91-106 × 106, 94-102 × 106, and 78-93 × 106, respectively. Preliminary analysis of restriction endonuclease profiles indicated a molecular weight of approximately 88 × 106 for the sum of the fragments. © 1979.

引用

页码：593 / 597

页数：5

共 25 条

[1] ANATOMY OF T5 BACTERIOPHAGE DNA MOLECULE [J].

ABELSON, J ;

THOMAS, CA .

JOURNAL OF MOLECULAR BIOLOGY, 1966, 18 (02) :262-&

[2]

BERGOLD G, 1947, Z NATURFORSCH B, V2, P122

[3]

Britten R J, 1974, Methods Enzymol, V29, P363

[4] REPEATED SEQUENCES IN DNA [J].

BRITTEN, RJ ;

KOHNE, DE .

SCIENCE, 1968, 161 (3841) :529-&

[5] DNA CONTAINED BY NUCLEAR POLYHEDROSIS VIRUSES ISOLATED FROM 4 SPODOPTERA-SPP (LEPIDOPTERA, NOCTUIDAE) - GENOME SIZE AND CONFIGURATION ASSESSED BY ELECTRON-MICROSCOPY [J].

BUD, HM ;

KELLY, DC .

JOURNAL OF GENERAL VIROLOGY, 1977, 37 (OCT) :135-143

[6] MOLECULAR-WEIGHTS OF LEPIDOPTERAN BACULOVIRUS DNAS - DERIVATION BY ELECTRON-MICROSCOPY [J].

BURGESS, S .

JOURNAL OF GENERAL VIROLOGY, 1977, 37 (DEC) :501-510

[7] SEDIMENTATION RATE AS A MEASURE OF MOLECULAR WEIGHT OF DNA [J].

BURGI, E ;

HERSHEY, AD .

BIOPHYSICAL JOURNAL, 1963, 3 (04) :309-&

[8]

DAVIS RW, 1971, METHOD ENZYMOL, V21, P413

[9] STUDIES ON ESCHERICHIA COLI SEX FACTORS .3. COVALENTLY CLOSED F'LAC DNA MOLECULES [J].

FREIFELD.D .

JOURNAL OF MOLECULAR BIOLOGY, 1968, 34 (01) :31-&

[10]

FREIFELDER D, 1970, Journal of Molecular Biology, V54, P567, DOI 10.1016/0022-2836(70)90127-0

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