P-31 RELAXATION-TIMES OF INORGANIC-PHOSPHATE AND NUCLEOTIDES IN AQUEOUS-SOLUTION

被引：11

作者：

GRANOT, J ^{[1
]}

ELGAVISH, GA ^{[1
]}

COHEN, JS ^{[1
]}

机构：

[1] WEIZMANN INST SCI,DEPT ISOTOPE RES,REHOVOT 76100,ISRAEL

来源：

JOURNAL OF MAGNETIC RESONANCE | 1979年 / 33卷 / 03期

关键词：

D O I：

10.1016/0022-2364(79)90168-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

31P spin-lattice relaxation times (T1) of inorganic orthophosphate, and of the nucleotides TMP, AMP and ATP are reported as a function of pH. The T1 values are found to exhibit significant pH dependences, with minimum T1 values generally observed at pH values close to the pKa values for the orthophosphates. The effects of the addition of Cu2+ or EDTA and treatment with cation exchange resin are described. These observations are related to similar results obtained previously for other ionizing species using 1H and 13C NMR, indicating a general phenomenon of such pH-dependent variations in T1 values in aqueous solutions. It is suggested that this effect stems from interactions with paramagnetic metal ion impurities. © 1979.

引用

页码：569 / 575

页数：7

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