INVITRO GENERATION OF NONSPECIFIC SUPPRESSOR CELLS AND THEIR CHARACTERIZATION

被引：22

作者：

ANACLERIO, A

MORAS, ML

HONORATI, C

RUGGERI, A

CONTI, G

SPREAFICO, F

机构：

[1] Laboratory of Immunology and Cancer Chemotherapy, Jstituto di Ricerche Farmacologiche “Mario Negri”, Milan, 20157

来源：

TRANSPLANTATION | 1979年 / 27卷 / 05期

关键词：

D O I：

10.1097/00007890-197905000-00008

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In vitro preculture of C3H/He splenocytes for 48 to 96 hr induces aspecific suppressor cells evaluated by their ability to reduce 3H-TdR uptake by fresh syngeneic splenocytes stimulated by different amounts of Concanavalin A (Con A) in vitro. This suppressive activity is obtained in medium containing 10% heat-inactivated fetal calf serum (FCS) or similar amounts of heat-inactivated bovine serum, syngeneic, or allogeneic murine sera but not by unheated FCS. Suppressive activity is resistant to mitomycin C and radiation up to 5,000 R. Exposure of precultured cells to carbonyl iron or plastic adherence, but not to anti-Thy 1.2 serum plus complement, results in removal of the suppressive activity. © 1979 by The Williams & Wilkins Co.

引用

页码：329 / 332

页数：4

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