CATALYTIC DETERMINATIONS OF ENZYMES AND METAL-IONS BY SAMPLE INJECTION IN CLOSED-LOOP FLOW SYSTEMS

Catalysts (enzymes or metal ions) can only be determined in closed-loop systems if, after signal detection, they are physically removed from the system or rendered inactive by an inhibitor. Successful removal of the enzyme glucose oxidase (by physical adsorption on phenoxyacetylcellulose traps) and copper ions (by controlled potential electrode-position) are described as examples leading to the determination of these catalysts by sample injection in closed-flow systems. The catalytic determination of copper combines a unique electrochemical removal of catalyst and simultaneous regeneration of the monitored species. © 1979, American Chemical Society. All rights reserved.