DETECTION OF HHV-6 BY THE POLYMERASE CHAIN-REACTION

被引：22

作者：

COLLANDRE, H ^{[1
]}

AUBIN, JT ^{[1
]}

AGUT, H ^{[1
]}

BECHET, JM ^{[1
]}

MONTAGNIER, L ^{[1
]}

机构：

[1] HOP PITTE SALPETRIERE,BACTERIOL VIROL,PARIS,FRANCE

来源：

JOURNAL OF VIROLOGICAL METHODS | 1991年 / 31卷 / 2-3期

关键词：

POLYMERASE CHAIN REACTION; VIRAL DIAGNOSIS; HHV-6;

D O I：

10.1016/0166-0934(91)90155-S

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The polymerase chain reaction (PCR) was used to detect human herpes virus 6 (HHV-6) sequences in tissue culture. A pair of primers was synthesized and used to amplify a conserved region of the genome. Amplified products were detected either by visualization of UV illuminated ethidium bromide stained gel or, by hybridization with a specific radiolabeled oligonucleotide. As little as 5 fg of HHV-6 could be detected in infected cells, making this assay suitable for diagnostic purposes.

引用

页码：171 / 180

页数：10

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