SEQUENCE-SPECIFIC DNA PURIFICATION BY TRIPLEX AFFINITY CAPTURE

被引：109

作者：

ITO, T

SMITH, CL

CANTOR, CR

机构：

[1] UNIV CALIF BERKELEY, DEPT MOLEC & CELL BIOL, 529 STANLEY HALL, BERKELEY, CA 94720 USA

[2] UNIV CALIF BERKELEY, LAWRENCE BERKELEY LAB, DIV CHEM BIODYNAM, BERKELEY, CA 94720 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 02期

关键词：

TRIPLE-HELIX DNA; BIOTINYLATED OLIGONUCLEOTIDE; STREPTAVIDIN; MAGNETIC SEPARATION; DINUCLEOTIDE REPEAT;

D O I：

10.1073/pnas.89.2.495

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A DNA isolation procedure was developed by using triple-helix formation and magnetic separation. In this procedure, target DNA is captured by a biotinylated oligonucleotide via intermolecular triplex formation, bound to streptavidin-coated magnetic beads, and recovered in double-stranded form by elution with a mild alkaline buffer that destabilizes the triple helix. The effectiveness of the procedure was demonstrated by a model experiment with an artificially reconstructed library and, also, by the isolation of (dT-dC)n.(dG-dA)n dinucleotide repeats from a human genomic library. This procedure provides a prototype for other triplex-mediated DNA isolation technologies.

引用

页码：495 / 498

页数：4

共 39 条

[1] 2ND STRUCTURAL MOTIF FOR RECOGNITION OF DNA BY OLIGONUCLEOTIDE-DIRECTED TRIPLE-HELIX FORMATION [J].