REACTIVE OXYGEN SPECIES PRODUCTION BY MONOCYTES AND POLYMORPHONUCLEAR LEUKOCYTES DURING DIALYSIS

被引：163

作者：

HIMMELFARB, J

LAZARUS, JM

HAKIM, R

机构：

[1] VANDERBILT UNIV,CTR MED,DIV NEPHROL,B-2214 MED CTR N,NASHVILLE,TN 37232

[2] UNIV VERMONT,SCH MED,MAINE MED CTR,DEPT MED,PORTLAND,ME

[3] HARVARD UNIV,SCH MED,BOSTON,MA 02115

[4] BRIGHAM & WOMENS HOSP,END STAGE RENAL DIS PROGRAM,BOSTON,MA 02115

来源：

AMERICAN JOURNAL OF KIDNEY DISEASES | 1991年 / 17卷 / 03期

关键词：

MONOCYTES; INTERLEUKIN; FLUORESCENCE; CUPROPHANE; C5A; F-MET-LEU-PHE;

D O I：

10.1016/S0272-6386(12)80473-2

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 [临床医学]; 100201 [内科学];

摘要：

Intradialytic production of reactive oxygen species (ROS) by monocytes and polymorphonuclear leukocytes (PMNL) was examined separately in six hemodialysis patients. Samples obtained 15 minutes after initiation of dialysis with new cuprophane membanes demonstrated significantly increased (P < 0.05) ROS production in both cell populations as measured by the fluorescence of a specific intracellular marker (2′,7′-dichlorofluorescein diacetate [DCFH-DA]) assayed by flow cytometry. Granulocytes harvested during dialysis also showed decreased responsiveness to exogenous C5a and F-Met-Leu-Phe (FMLP) at 15, 30, and 60 minutes after initiation of dialysis (P < 0.05). Our data suggest that hemodialysis with cuprophane membrane is associated with monocyte and PMNL activation as shown by production of ROS coincident with peak activation of the complement cascade; these granulocytes become refractory to further stimulation with C5a and FMLP during dialysis. © 1991, National Kidney Foundation, Inc.. All rights reserved.

引用

页码：271 / 276

页数：6

共 28 条

[1]

BASS DA, 1983, J IMMUNOL, V130, P1910

[2]

CUPRAMMONIUM MEMBRANES STIMULATE INTERLEUKIN-1 RELEASE AND ARACHIDONIC-ACID METABOLISM IN MONOCYTES IN THE ABSENCE OF COMPLEMENT [J].