HYBRIDOMA GROWTH AND MONOCLONAL-ANTIBODY PRODUCTION IN IRON-RICH PROTEIN-FREE MEDIUM - EFFECT OF NUTRIENT CONCENTRATION

被引：48

作者：

FRANEK, F

DOLNIKOVA, J

机构：

[1] Department of Fundamental Cytotechnology, Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha 4

来源：

CYTOTECHNOLOGY | 1991年 / 7卷 / 01期

关键词：

MOUSE-MOUSE HYBRIDOMA; PROTEIN-FREE SUPPLEMENT; BASAL MEDIUM; APOPTOSIS; BIOREACTOR CULTURE;

D O I：

10.1007/BF00135636

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The iron-rich (500-mu-M ferric citrate) protein-free supplement was added to six different basal media. Cell growth and monoclonal antibody production of a mouse-mouse hybridoma were investigated in 1.3 1 batch cultures performed in a laboratory bioreactor with automatic control of pH and dissolved oxygen concentration. RPMI 1640 served as the control medium. Fortification of the basal medium by balanced mixtures of amino acids and vitamins showed higher positive effect than daily supplementation by glucose and glutamine. Strongly fortified medium, based on RPMI 1640, was found superior to other basal media. The viability index increased by a factor of 3.04 and the total antibody production by a factor of 2.82, relative to the control.

引用

页码：33 / 38

页数：6

共 12 条

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