FLUORESCENCE STUDIES OF A LOCAL-ANESTHETIC PHOSPHOLIPID INTERACTION

被引：14

作者：

BARGHOUTHI, S ^{[1
]}

EFTINK, MR ^{[1
]}

机构：

[1] UNIV MISSISSIPPI,DEPT CHEM,UNIVERSITY,MS 38677

来源：

BIOPHYSICAL CHEMISTRY | 1993年 / 46卷 / 01期

关键词：

DIBUCAINE; PHOSPHOLIPIDS; UNILAMELLAR VESICLES; FLUORESCENCE QUENCHING; FLUORESCENCE LIFETIME; FLUORESCENCE ANISOTROPY DECAY;

D O I：

10.1016/0301-4622(93)87002-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Steady-state and time-resolved fluorescence data are reported for the local anesthetic dibucaine in the absence and presence of phospholipid vesicles. These vesicles were comprised of dimyristylphosphatidyl choline and approximately 10% dimyristylphosphatidyl glycerol. Solute quenching studies show the bound drug to be protected from collision with iodide ion. The fluorescence lifetime of dibucaine is not significantly changed upon binding to vesicles. The fluorescence anisotropy of dibucaine increases upon association with the vesicles. Anisotropy decay measurements show that the rotational correlation time, psi, of bound dibucaine is increased about one hundred fold over that for free dibucaine. This indicates that the rotational motion of bound dibucaine is slowed by its interaction with the phospholipids. However, we find no evidence that the rotational motion of bound dibucaine is anisotropic.

引用

页码：13 / 19

页数：7

共 29 条

[1] HOST GUEST INTERACTIONS - A FLUORESCENCE INVESTIGATION OF THE SOLUBILIZATION OF DIPHENYLPOLYENE SOLUTE MOLECULES IN LIPID BILAYERS [J].