ANALYSIS OF HUMAN ANTIVIRAL CYTOTOXIC T-LYMPHOCYTE RESPONSES FOR VACCINE TRIALS USING CRYOPRESERVED MONONUCLEAR LEUKOCYTES - DEMONSTRATION OF FEASIBILITY WITH INFLUENZA VIRUS-SPECIFIC RESPONSES

被引：4

作者：

ELDAHER, N ^{[1
]}

NICHOLS, JE ^{[1
]}

ROBERTS, NJ ^{[1
]}

机构：

[1] UNIV ROCHESTER,MED CTR,SCH MED,DEPT MED,INFECT DIS UNIT,ROCHESTER,NY 14642

来源：

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY | 1994年 / 1卷 / 05期

关键词：

D O I：

10.1128/CDLI.1.5.487-492.1994

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The feasibility of measuring virus-specific human cytotoxic T-lymphocyte (CTL) activity by using cryopreserved mononuclear leukocytes to support clinical vaccine trials was addressed. Autologous fresh and cryopreserved cells from the same sample of peripheral blood were used as sources of CTL precursors and were tested for influenza virus-specific activity. The data indicated that virus-specific CTL activity could be measured by using cryopreserved cells; this could also be done in assays that are designed to characterize the responsible effector cell population.

引用

页码：487 / 492

页数：6

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